The skills of physical assessment and examination provide nurses with powerful tools to detect subtle, as well as obvious changes in a client’s health. Physical assessment enables the nurse to assess patterns reflecting health problems and to evaluate the client’s progress following a treatment therapy1. This study was conducted with an objective To Assess the knowledge regarding selected physical assessments scales used in Cardiac and Central Nervous system in adult patient among staff Nurses by using structured knowledge questionnaire. Methodology: Non experimental Descriptive research design was used. By using simple random sampling technique 50 staff Nurses were selected and assessed their Knowledge by using self administered structured Knowledge questionnaire at R.L.J.Hospital and Research Centre. Results: The knowledge level of study participants was moderate 9(18%) and none of the samples belongs to adequate knowledge level. Conclusion: The overall knowledge scores reveals that majority of staff Nurses were having inadequate knowledge on use of assessment scales, thus it lead to wrong decision, Diagnosis and inappropriate care which endangers the life of the client.