Whole blood is still commonly transfused in developing countries including India where blood components are not easily available.The haematological and biochemical changes associated with the storage of blood in our blood banks have been reported. Blood (350 ml) was drawn from 65 healthy volunteer donors into CPDA–1 anticoagulant and placed on the quarantine shelf of the blood bank refrigerator maintained at 2–6°C. Blood bags were screened for HCV, HBsAg, Syphilis, Malaria and HIV 1&2 and were confirmed negative. Samples were collected at 1, 7, 14, 21, 28 and 35 days and tested for hematological and biochemical.