Staphylococcus aureus is amongst the most dangerous human pathogens, including bacteremia, pneumonia, osteomyelitis, meningitis, etc. The overuse of antibiotics and incompliance with the drug courses have resulted into antibiotic resistance amongst these microorganisms. Present study demonstrates the changing trends in antibiotic susceptibility amongst the Staphylococcus aureus species isolated from pus samples from various clinical units in a tertiary care hospital. A retrospective study was conducted for a period of 2 years for the isolation of S.aureus species. Various antibiotics were tested against S.aureus based on CLSI guidelines. A total of 275 S.aureus strains were isolated out of which 163 (59%) were Methicillin–resistant Staphylococcus aureus (MRSA). A rise in the occurrence of Methicillin–sensitive Staphylococcus aureus (MSSA) has also been observed with an increase in resistance to various antibiotics amongst them. S.aureus showed highest sensitivity to Vancomycin (30µg) followed by Gentamicin (10µg) and Cotrimoxazole (1.25/23.75 µg).