Background: This study aimed to investigate the bacteria associated with urinary tract infection (UTI) and antibiotic susceptibility pattern of the isolates during LAST one year in a tertiary care hospital of central india.

Materials and Methods: Overall 380 patients attending OPD with clinical symptoms of UTI were subjected as samples, and they were cultured and pure isolated bacteria were identified using biochemical tests and subjected to antibiogram assessment using disc diffusion method.

Results: Totally, 57 (15%) from 380 patients had positive UTI result. 51 (89.5% of positive case) patients were resistant to at least one antibiotic.  Escherichia coli, Staphylococcus spp., and Pseudomonas spp. were the most prevalent bacteria which were present in 25(43.8%), 17(29.8%)and 5(8.7%) of the positive samples, respectively.

Conclusion: The results of this study revealed a great concern for emerging UTI–related multidrug–resistant strains of bacteria causing UTI in Iran.